Identification, introduction, ecology and ethnobotany
M. Ghorbani; S. Khorasaninejad; Kh. Hemmati; Kh. Ghorbani
Abstract
This study was aimed at comparing Viola spp. populations collected from north and northwest of Iran to investigate the adaptability and feasibility of their domestication under climatic conditions of Gorgan. The whole plant samples together with their rhizomes were collected from nine habitats in Golestan, ...
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This study was aimed at comparing Viola spp. populations collected from north and northwest of Iran to investigate the adaptability and feasibility of their domestication under climatic conditions of Gorgan. The whole plant samples together with their rhizomes were collected from nine habitats in Golestan, Mazandaran, Gilan, East Azerbaijan, and Kermanshah provinces during the growing season. After botanical approval and receiving herbarium code, the rhizomes were cultivated under the same soil and climate with three replications. Their yield components and physiological, morphological, and phytochemical traits were measured after one crop year at flowering stage. The results showed that these populations were from V. alba Besser, V. odorata L., V. sieheana W.Becker, and V. ignobilis Rups. species. All populations produced flowers and seeds after one crop year. The populations differed significantly (P<0.01). V. ignobilis from Kermanshah had the highest amount of root and aerial parts fresh weight, aerial parts dry weight, and leaf area. The highest amount of root and aerial parts length belonged to V. sieheana from Alangdare. V. alba from Ziarat had the highest amount of total phenols (45.74 mg gallic acid per gram of dry extract) and total flavonoids (20.24 mg quercetin per gram of dry extract). The highest amount of chlorophylls a and b, total chlorophyll, carotenoids, and anthocyanins was observed in V. odorata from Bandargaz. The highest antioxidant activity by DPPH and ABTS methods was found in V. alba from Afratakhte and the highest one by FRAP was observed in V. odorata from Qarnabad. The highest amount of total antioxidant was measured in V. alba and V. oderata populations from Ziarat region. Overall, the results proved the considerable traits diversity in Viola spp. populations. This diversity could be due to the high genetic potential among different populations, differences in environmental conditions, or the interaction of population and environment.
H. Nouri Dashlibroon; S. Khorasaninejad; S.J. Mousavizadeh; M.H. Mirjalili
Abstract
number of sub leaves (leaves on sub-branches), number of internodes on main stems, number of lateral stems, spike length on the main panicle, number of spikes, number of flowers per panicle, fresh and dry weight of aerial parts, fresh and dry weight of roots, root length, leaf area, internode length, ...
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number of sub leaves (leaves on sub-branches), number of internodes on main stems, number of lateral stems, spike length on the main panicle, number of spikes, number of flowers per panicle, fresh and dry weight of aerial parts, fresh and dry weight of roots, root length, leaf area, internode length, total phenol content, total flavonoids content, antioxidant activity, total sugar content and chlorophyll quality were measured. The results of the analysis of variance showed that the studied treatments had a significant effect on all traits except the number of main leaves, the number of internodes on main stems, and root length. The means comparison showed that with increasing the concentration and period of colchicine application, height of aerial parts, internode length, the number of flowers per panicle, and total sugar content decreased and spike length on the main panicle and total phenol content increased. Based on the results of flow cytometric analysis, the mean genome size was 0.73 pg in plants treated with colchicine at a concentration of 0.4% for 48 hours, while the mean genome size in the control plants was 0.55 pg. Plants in other treatments showed a mean genome size of 0.57. In general, the results of this experiment showed a positive response of L. stricta to colchicine for the induction of polyploidy and the possibility of using this method in the future breeding of this plant.
M. Mazloomi Abukhyly; S. khorasaninejad; M. Alizadeh
Abstract
Lavandula stricta Del. is a native aromatic plant in Iran from Lamiaceae, traditionally used for treatment of rheumatic and stomach pain and cough. In usual agricultural systems, the purpose of using fertilizers and their combination is to obtain the highest seed yield and quality of the active substances. ...
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Lavandula stricta Del. is a native aromatic plant in Iran from Lamiaceae, traditionally used for treatment of rheumatic and stomach pain and cough. In usual agricultural systems, the purpose of using fertilizers and their combination is to obtain the highest seed yield and quality of the active substances. This study was aimed to compare the effects of nanofertilizer and fertilizer on growth indices, morphological and biochemical properties of lavender "Upright" (Lavandula stricta Del.) regenerated from seed and tissue culture. The experiment was conducted as factorial design based on randomized complete block design with three replications and four experimental units at the research farm of Gorgan University of Agricultural Sciences and Natural Resources, during the growing season of 2016-2017. The treatments were two plants with different regeneration methods (seeds and tissue culture) and various types of fertilizers (control or no fertilizer, NPK fertilizer at concentrations of 10 and 20 kg per hectare and nanofertilizer at concentrations of 10 and 20 kg ha). The results showed that fertilizer treatments had significant effects on all measured properties except leaf area, shoot height, internodes length, shoot fresh and dry weight, 1000-seed weight and total phenol content (P<0.01). The type of plant also caused differences in biochemical, developmental and morphological properties except shoot height, number of main stem, shoot fresh and dry weight, and 1000-seed weight. Interaction effect of fertilizer and type of plant was significant for all measured properties except shoot height, shoot fresh weight and 1000-seed weight. In general, the results showed that application of NPK nano fertilizer had positive effect on the growth of tissue culture plants and resulted in improved yield.
S. Chorli; S. Khorasaninejad
Abstract
The biological control of plant pathogens is a priority for reducing of the dangerous effects of chemical pesticides that are include threats for human health, environmental pollution, and the emergence of pathogens resistant eliminates. Ceratocephalus falcatus L. such a buttercup, plant is very small ...
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The biological control of plant pathogens is a priority for reducing of the dangerous effects of chemical pesticides that are include threats for human health, environmental pollution, and the emergence of pathogens resistant eliminates. Ceratocephalus falcatus L. such a buttercup, plant is very small annual plant, 3-5 cm tall, it has short stems. Plant samples were collected based on completely randomized design with three replications in May 2015 from the village of Buzhmehran (city of Neyshabur) to record of morphological, phytochemical characterization such as total phenol, total flavonoids and antioxidant water extract, methanol and acetone plant the fungus Fusarium graminearum. The number of flowers per plant on average 5 numbers, leaf number was 19 and the plant is 3 cm tall. The total phenol Gallic acid 0/098 mg per gram of dry weight, the flavonoids quercetin 0/302 mg per gram of dry weight and percent free radical scavenging antioxidant activity it was determined 70/618. The effect of the extracts on the fungus Fusarium graminearum were showed that the most-linear growth of mycelium to control the methanol extract had the lowest. As a result, methanol extracts percent more on linear growth inhibition was assessed fungus can be concluded that Ceratocephalus falcatus has antifungal properties and enjoys good standing in study programs.
H. Sanginabadi; S. Khorasaninejad; Kh. Hemmati; A. Ghasemnejad
Abstract
Lavandula stricta Del. is a native aromatic plant in Iran from Lamiaceae family, traditionally used in the treatment of rheumatic pain, nausea, and flu. In order to investigate the common propagation methods in this plant, two experiments were designed to evaluate the effect of different treatments on ...
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Lavandula stricta Del. is a native aromatic plant in Iran from Lamiaceae family, traditionally used in the treatment of rheumatic pain, nausea, and flu. In order to investigate the common propagation methods in this plant, two experiments were designed to evaluate the effect of different treatments on seed germination and rooting of cuttings at the Gorgan University of Agricultural Sciences and Natural Resources. The first experiment was carried out in a factorial experiment based on a completely randomized design (CRD) with three replications, and two factors were included as follows: Gibberlic acid (0, 250, 500 and 750 ppm) and chilling (chilling at 4 °C for 24 and 48 hours and chilling at -18 °C for 24 and 48 hours). The second experiment was carried out in a factorial experiment based on a complete randomized blocks design with three replications and three factors including indole butyric acid (0 and 250ppm), two types of cutting and three types of medium (cocopeat + perlite, perlite+sand and cocopeat+sand). Then, rooting percentage, root length and root number were measured. According to the results of the first experiment, chilling treatments caused to the reduced germination percentage, seed vigor, plumule and radicle length. In addition, gibberellic acid reduced all seed growth properties but increased the root length. In the second experiment, results showed that the highest rooting (60%) was obtained in the medium containing perlite+sand and 250 ppm ABA.
